Sigma-Aldrich
Trizma Base Bioperformance Certified Cell 99%, 5 kg
- Part Number:
- MIL-T6066-5KG
- Lead Time:
- 7-14 days
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- Quantity:
- each
- Size:
- 5 kg
- Style:
- chemical
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Description
Trizma Base Bioperformance Certified Cell 99%, 5 Kg
Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
- CAS Number 77-86-1
- Linear Formula NH2C(CH2OH)3
- Molecular Weight 121.14
- Beilstein/REAXYS Number 741883
- EC Number 201-064-4
- MDL number MFCD00004679
- PubChem Substance ID 24900345
- NACRES NA.25
| Quality Level | 400 |
| grade | BioPerformance Certified |
| description | aminopeptidase substrate |
| assay | ≥99.9% (titration) |
| form | crystalline powder |
| application(s) | cell culture | mammalian: suitable |
| impurities | DNase, RNase, protease, none detected |
| Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested | |
| ≤0.2% water (Karl Fischer) | |
| loss | ≤0.5% (loss on drying, 110 °C) |
| pH | 10.0-11.5 |
| useful pH range | 9-Jul |
| pKa (25 °C) | 8.1 |
| bp | 219-220 °C/10 mmHg (lit.) |
| mp | 167-172 °C (lit.) |
| solubility | water: soluble (666 mg/ml, yielding a clear colorless solution) |
| absorption | ≤0.05 at 260 at 1 M |
| ≤0.05 at 290 at 40% | |
| ≤0.06 at 280 at 1 M | |
| suitability | meets EP, USP testing specifications |
| Featured Industry | Diagnostic Assay Manufacturing |
| SMILES string | NC(CO)(CO)CO |
| InChI | 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2 |
| InChI key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
General description
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
Application
Trizma has been used:
• As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
• As a component of pre-hybridization buffer in in situ hybridization
• To suspend dried protein before SDS-PAGE protein characterization
• As a component of lysis buffer to wash and lyse cells before immunoprecipitation
• In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
• In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
• In capillary electrochromatography and UV analysis of tocopherols and tocotrienols
Safety Information
RIDADR NONH for all modes of transport
WGK Germany 1
RTECS TY2900000
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
Most chemicals cannot be expedited.
WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.
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