Sigma-Aldrich

Triton® X-100 BioXtra, 10 Liter

Part Number:
MIL-T9284-10L
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2-4 weeks
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Size:
10 liter
Style:
chemical
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Price: $1,059.00

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Description

Triton X-100 Bioxtra, 10 Liters. A common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Synonym: t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

  • CAS Number 9002-93-1
  • Linear Formula t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
  • Beilstein/REAXYS Number 2315025
  • MDL number MFCD00128254
  • PubChem Substance ID 329826903
  • NACRES NA.21

Applications

  • Along with Span® 80 and Tween® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
  • As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
  • In the blocking solution during immunocytochemistry procedure
  • During deglycosylation procedure
  • For membrane solubilization
  • For cell permeabilization
  • In decellularization of porcine heart valve tissue

Specifications

  • biological source: synthetic:
  • Quality Level: 200
  • Description: non-ionic
  • form:liquid
  • molecular weight: micellar avg mol wt 80,000 / average mol wt 625
  • aggregation number: 100-155:
  • technique(s): permeability assay: suitable
  • impurities: ≤0.05% Phosphorus (P)
  • ign. residue: ≤0.4%
  • CMC: 0.2-0.9 mM (20-25°C)
  • transition temp: cloud point 65°C / pour point ~7°C
  • solubility: water: soluble
  • density: 1.065 g/cm3 at 20°C (68 °F)
  • HLB: 13.5
  • SMILES string: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
  • InChI1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5p
  • InChI key HNLXNOZHXNSSPN-UHFFFAOYSA-N

anion traces:

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces:

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.1%
Pb: ≤0.001%
Zn: ≤0.0005%

Safety Information

Signal word: Danger
Hazard statements: p02-p15-p18-H410
Precautionary statements: P280-P301 + P312 + P330-P305 + P351 + P338 + P310
Personal Protective Equipment: Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
RIDADR UN 3082 9 / PGIII
WGK Germany 2
RTECS MD0907700
Flash Point(F): 483.8 °F
Flash Point(C): 251 °C

Documents

Safety Data Sheet

Warnings

Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.

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WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning

Please note: This product is not returnable.

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MPN:
T9284-10L