Research Antibiotics, Sulfadiazine, 100 grams

Sulfadiazine, 100 gram. Sulfadiazine is a sulfonamide antibiotic that was developed in 1940 by Richard Roblin at the Stamford Research Laboratories of the American Cyanamid Company to be a more potent and less toxic alternative to Sulfapyridine. Sulfadiazine is a bacteriostatic folic acid synthesis inhibitor that shows broad spectrum activity against gram-positive bacteria, gram-negative bacteria and Chlamydia.

Sulfadiazine is a competitive inhibitor of the bacterial enzyme dihydropteroate synthetase. This enzyme is needed for the proper processing of para-aminobenzoic acid (PABA) which is essential for folic acid synthesis. The inhibited reaction is necessary in these organisms for the synthesis of folic acid. Sulfadiazine is sparingly soluble in aqueous solution (0.077 mg/mL).

Synonyms: 4-Amino-N-(2-pyrimidinyl)benzenesulfonamide, N1-(Pyrimidin-2-yl)sulfanilamide

• Category: Microbiology
• CAS Number: 68-35-9
• Molecular Formula: C₁₀H₁₀N₄O₂S
• Molecular Weight: 250.28 g/mol
• Mechanism of Action: Sulfadiazine is a competitive inhibitor of the bacterial enzyme dihydropteroate synthetase. This enzyme is needed for the proper processing of para-aminobenzoic acid (PABA) which is essential for folic acid synthesis. The inhibited reaction is necessary in these organisms for the synthesis of folic acid.
• torage Conditions: 2-8°C
• Tariff Code: 2935.00.4200
• Spectrum: Sulfadiazine is a broad-spectrum antibiotic targeting a wide range of gram-positive and gram-negative bacteria especially those which cause urinary tract infections.

Specifications

• Form: Powder
• Appearance: White or almost white crystalline powder.
• Source: Synthetic
• Impurity Profile:
  • Impurity A| Pyrimidin-2-amine|109-12-6|C4H5N3|95.1|
  • Impurity B| 4-Aminobenzenesulfonic Acid (Sulphanilic Acid)|121-57-3|C6H7NO3S|173.19|
  • Impurity D| 4-Aminobenzenesulfonamide (Sulfanilamide)|63-74-1|C6H8N2O2S|172.20|
  • Impurity UN1| N4-Acetylsulfadiazine|5572-57-6|C12H12N4O3S|292.31|
• Assay (on dried basis): 99.0% - 101.0%
• Loss on Drying: Less than 0.5%
• Residue On Ignition: Less than 0.1%
• Heavy Metals: Less than 10 ppm

Microbiology Applications

Sulfadiazine is commonly used in clinical in vitromicrobiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

• Neisseria meningitidis 0.5 µg/mL – 200 µg/mL

Sulfadiazine is a component of SPS agar (Sulfite Polymyxin Sulfadizine Agar), that can be used for the detection, isolation, and emuneration of Clostridium perfringens and Clostridium botulinum in food; as well as the detection of V. cholerae and V. vulnificus in the environment.

Sulfadiazine is commonly used with pyrimethamine to treat toxoplasmosis in patients with acquired immunodeficiency syndrome. It is also used to treat newborns with congenital infections. Sulfadiazine has been used to control acute infections when studying murine models of reactivated toxoplasmosis.

Plant Biology Applications

Sulfadiazine is a herbicide that is able to inhibit folic acid synthesis in plants. Sulfadiazine and the resistance gene sul, create an effective selection system for the creation of transgenic tobacco and algae. Co-transformation experiments in tobacco revealed that sul is even superior to nptII, the currently most efficient selectable marker gene, and thus provides an attractive marker for the high‐throughput genetic transformation of plants and algae.

References

"Sulfadiazine: Mechanism of Action." Cancer News Network. N.p., 2009. Web. 21 Aug. 2012.

Tabatabaei, I., Dal Bosco, C., Bednarska, M., Ruf S., Meurer, J., Bock, R. (2018) A highly efficient sulfadiazine selection system for the generation of transgenic plants and algae. Plant Biotechnol. J., https://doi.org/10.1111/pbi.13004

Minden, V., Deloy, A., Volkert, A. M., Leonhardt, S. D., & Pufal, G. (2017). Antibiotics impact plant traits, even at small concentrations. AoB PLANTS, 9(2), plx010. doi:10.1093/aobpla/plx010

Available Documents

• SDS
• Product Data Sheet
• Susceptibility and Resistance Data

Made by an ISO 9001:2015 certified antibiotic manufacturer focused on creating antibiotics through fermentation.

By removing contamination and streamlining gene selection, these research antibiotics help scientists make discoveries in cell/plant biology, microbiology, virology, stem cell therapy, and cancer research. Specializing in the pharmaceutical and biotech research sectors.

These products are specifically designed for research use and are not approved for human or animal consumption. Not FDA approved.

Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not ship to a registered laboratory or research facility.

Please note: This product is not returnable.