Research Antibiotics, Amphotericin B, USP, 500 mg

Amphotericin B, USP, 500 mg. This is a polyene antifungal or antimycotic compound derived from Streptomyces nodosus. It is used to control contamination from fungi, viruses and protozoa.

We offer 3 forms of Amphotericin B:

• Amphotericin B, EP (TOK-A064)
• Amphotericin B, USP (TOK-A007)
• Amphotericin B, solubilized (TOK-A008)

The compound is nearly insoluble in water at pH 6-7 (but is soluble at pH 2 or 11). It is soluble in dimethyl sulfoxide and dimethylformamide. Amphotericin B, USP conforms to United States Pharmacopoeia specifications.

Description

• Family: Microbiology
• CAS Number: 1397-89-3
• Molecular Formula: C₄₇H₇₃NO₁₇
• Molecular Weight: 924.08
• Mechanism of Action: Amphotericin B associates with membrane sterols (ergosterol in fungal cell membranes, and cholesterol in mammalian cell membranes). Amphotericin B forms a pore in these membranes resulting in leakage of essential ions and ultimately cell death.
• Storage Conditions: 2-8°C
• Tariff Code: 2941.90.1050
• Spectrum: Amphotericin B is active against mammalian cells, fungi, viruses, and protozoa. Amphotericin B is not toxic to bacteria due to their lack of sterols. The following represents MIC susceptibility data for amphotericin B against common fungal pathogens:
  • Candida albicans - 0.001 - 321 µg/mL
  • Candida krusei - 0.001 - 16 µg/mL
  • Coccidioides immitis - 0.0625 - 2 µg/mL
  • Cryptococcus neoformans - 0.2 - 39 µg/mL
  • Fusarium oxysporum - 0.75 - 125 µg/mL

Specifications

• Form: Powder
• Appearance: Yellow or Orange hygroscopic powder
• Source: Streptomyces nodosus
• Water Content (Karl Fischer): ≤5.0%
• Assay (On Dried Basis): ≥750 IU/mg
• Residue on Ignition: ≤3.0%
• Loss on Drying ≤5.0%
• Impurities
  • Amphotericin A: ≤15.0%
• Endotoxin: ≤0.9 EU/mg

Eukaryotic Cell Culture Applications

Amphotericin B can be used in eukaryotic cell culture to control or prevent contamination from fungi, viruses, and protozoa. Amphotericin B can be toxic to cell lines at high concentrations and should not be used at concentrations greater than 2.50 µg/mL. Amphotericin B has been used in in vitro to inhibit the generation of the scrapie isoform of the prion protein when studying transmissible spongiform encephalopathies (Mangé et al 2000).

Microbiology Applications

Amphotericin B is used as an antimycotic selective agent in several routinely used selective media formulations to inhibit the growth of background fungal growth. It can also combat viruses and protozoa.

Plant Biology Applications

Amphotericin B can be used to inhibit phytopathogenic fungi in vitro

Available Documents

• SDS
• Product Data Sheet

Made by an ISO 9001:2015 certified antibiotic manufacturer focused on creating antibiotics through fermentation.

By removing contamination and streamlining gene selection, these research antibiotics help scientists make discoveries in cell/plant biology, microbiology, virology, stem cell therapy, and cancer research. Specializing in the pharmaceutical and biotech research sectors.

These products are specifically designed for research use and are not approved for human or animal consumption. Not FDA approved.

Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not ship to a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.

Most chemicals cannot be expedited.

Please note: This product is not returnable.