Sigma-Aldrich
PIPES 99% Titration, 100 grams
- Part Number:
- MIL-P6757-100G
- Lead Time:
- 2-3 weeks
- Minimum Purchase:
- 1 unit
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- each
- Size:
- 100 grams
- Style:
- chemical
Bulk discount rates
Automatically save when you buy in bulk.
| Buy 6 - 11 | and get 10% off |
| Buy 12 or above | and get 15% off |
Description
PIPES ≥99% Titration 100 Gram
Synonym: 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)
- CAS Number 5625-37-6
- Empirical Formula (Hill Notation) C8H18N2O6S2
- Molecular Weight 302.37
- Beilstein/REAXYS Number 817713
- EC Number 227-057-6
- MDL number MFCD00006159
- PubChem Substance ID 24898793
- NACRES NA.25
| Quality Level | 300 |
| assay | ≥99% (titration) |
| useful pH range | 6.1 - 7.5 |
| pKa (25 °C) | 6.8 |
| mp | >300 °C (lit.) |
| solubility | 1 M NaOH: 20 + 80 mL g, clear, colorless |
| Featured Industry | Diagnostic Assay Manufacturing |
| SMILES string | OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O |
| InChI | 1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16) |
| InChI key | IHPYMWDTONKSCO-UHFFFAOYSA-N |
General description
PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.
Application
Glutaraldehyde fixation of plant and animal tissue samples can cause loss of lipid, leading to apparent morphological changes. Lipid loss and artifacts were minimized when PIPES was used to buffer the glutaraldehyde fixative.,
Alkaline phosphatase activity is lost selectively from certain rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde. When PIPES was used as buffer, retention of activity was 60% greater.
Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer.
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
Most chemicals cannot be expedited.
This item has a Haz-Mat shipping fee built into the price. Fee is applicable only to the first item purchased. Additional HazMat items will be discounted automatically in the shopping cart. HazMat items always require special DOT handling and in rare cases may incur additional fees not levied prior to checkout. Request a quote for the most accurate pricing.
WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.