Nalgene® 5100-0001 Mr. Frosty Freezing Container for 1.2-2mL CryoVials, Polycarbonate

Freeze cells in tubes from 1.2 to 2mL using the Nalgene 5100-0001 Mr. Frosty trade; Freezing Container. This Cryo Freezing Container is inexpensive, reusable and convenient for freezing biological samples at the recommended controlled rate of cooling, -1°C/minute. Isopropyl alcohol is necessary to achieve the recommended rate of cooling. The simple to use system is designed to achieve a rate of cooling very close to the optimal rate for cell preservation. No contact with the alcohol: no wicking, no label removal and no compromise of tube hand printing.

Saves precious freezer space during the cooling process. Screw top lid secures samples inside in the event of accidental container tipping. Eliminates unreliable, improvised foam container devices.

Product Dimensions (H x Dia) : 86mm x 117mm
Tube Compatibility: 1.0 to 2.0mL

Requires: 100% Isopropyl Alcohol and a mechanical freezer.

Dimensions:

• Height (Metric): 86 mm
• Diameter (Metric): 117 mm

Specifications:

• Capacity (Metric): 2 mL
• Color: Clear, Blue Lid
• Holds: 18 (1.0 to 2.0mL Tubes)
• Material: Polycarbonate
• Temperature Range: -1°C
• Type: Freezing Container

Product Contains: Polycarbonate container, high-density polyethylene closure, tube holder, foam insert.

DIRECTIONS:
1. Remove high-density polyethylene tube holder and foam insert from polycarbonate unit. Discard foam insert.
2. Add 100% isopropyl alcohol to the fill line on the Mr. Frosty container. DO NOT overfill.
3. Carefully replace tube holder.
4. Place tubes containing sample into holes in tube holder.
5. Place unit in bottom of -70°C to -80°C mechanical freezer. Leave undisturbed for a minimum of 4 hours.
6. Remove frozen tubes from unit and place in a permanent, long-term storage freezer (e.g. -130°C or below.)

NOTE: Do not store full jar on its side. Store at room temperature when not in use. Replace alcohol every fifth use. Clean with non-abrasive, non-alkaline detergent such as Thermo Scientific Nalgene L-900 Detergent (Cat. No. 900-4000.) The tube holder floats with tubes in place and can be used as a floating rack when thawing samples.

Made in Mexico.

Frequently asked questions (FAQs)

At what temperature should my Mr. Frosty controlled rate cell freezing container be when I start freezing cells?

For best cooling rate performance, the entire system should be at room temperature just prior to freezing.

Should I retain or discard the foam insert in my Mr. Frosty controlled rate cell freezing container?

The white foam insert serves no purpose other than to make the printed instructions on the jar easier to read. Its presence or absence will not affect the cooling rate, and so it can be discarded.

Documents

Manuals & protocols
Nalgene Mr. Frosty Cryo 1°C Freezing Containers Package Insert