Sigma-Aldrich
Chaps Hydrate 98% HPLC, 100 grams
- Part Number:
- MIL-C3023-100G
- Lead Time:
- 2-4 weeks
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- each
- Size:
- 100 grams
- Style:
- chemical
- DOT Rated:
- HazMat Transport Fee Required
Bulk discount rates
Automatically save when you buy in bulk.
| Buy 6 - 11 | and get 10% off |
| Buy 12 or above | and get 15% off |
Description
Chaps Hydrate ≥98% HPLC 100 Gram
Synonym: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
- CAS Number 331717-45-4
- Empirical Formula (Hill Notation) C32H58N2O7S · xH2O
- Molecular Weight 614.88 (anhydrous basis)
- PubChem Substance ID 24892560
- NACRES NA.21
| Quality Level | 400 |
| description | zwitterionic |
| assay | ≥98% (HPLC) |
| mol wt | 614.88 g/mol (anhydrous basis) |
| application(s) | dialysis: suitable |
| electrophoresis: suitable | |
| CMC | 8 mM |
| mp | 157 °C (dec.) (lit.) |
| transition temp | cloud point >100 °C |
| SMILES string | O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C |
| InChI | 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1 |
| InChI key | SJCUTFKCLFLIFE-JWTJKVBLSA-N |
General description
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.
Application
0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion. 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction. 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis. CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS.
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
Most chemicals cannot be expedited.
This item has a Haz-Mat shipping fee built into the price. Fee is applicable only to the first item purchased. Additional HazMat items will be discounted automatically in the shopping cart. HazMat items always require special DOT handling and in rare cases may incur additional fees not levied prior to checkout. Request a quote for the most accurate pricing.
WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.