C18-W Silica TLC Plates, w/UV254, Aluminum Backed, 150um, 4x8cm, pack/50

C18-W TLC Plates with Octadecyl-Modified Silica Layers. Our C18-W plates feature a partial octadecyl (C18) modification of the silica with a carbon content of 14%. They can be used in Normal Phase or Reversed-Phase separation modes with eluents from anhydrous solvents to mixtures with high concentrations of water.

Features:

• Order of polarity: Silica > DIOL > Np > CN > C2 > C18-50 > C18-W > C18-100
• Wettable with water, can be used in Normal or Reversed Phase modes 

C18 -W TLC Plates Specifications:

• Silica Gel Technical Specifications:
  • Mean pore diameter: 60A
  • Specific surface area: 500 m2/g
  • Specific pore volume: 0.75 ml/g
  • Particle size
    • Analytical: 2-10µm
    • Preparative: 5-17µm
• Substrate:
  • Glass
  • Aluminum
• Layer Thickness
  • Analytical: 200μm
  • Preparative: 1000µm
• pH stability 2-10
• Indicator: acid-resistant product with a pale blue fluorescence for short-wave UV (254 nm)
• UV-absorbing substances appear as dark-blue to black spots on a light – blue background

Recommended Applications:

• Aminophenols, barbiturates, preservatives, nucleobases, polycyclic aromatic hydrocarbons, steroids, tetracyclines, plasticizers (phthalates)

Normal Phase TLC Plates: Durable, High-Quality Plates Improve Separation Efficiency and Reproducibility

Normal phase chromatography is a chromatographic type that uses polar stationary phases and nonpolar mobile phases. Sorbtech offers a wide selection of normal phase TLC plates for numerous normal phase applications.

Sorbtech Normal Phase TLC Plates Feature:

• A comprehensive range of plate sizes, surface chemistries, and three available backings
• Pre-coated plates ready for immediate use
• Available with or without UV Indicator
• Homogeneous, smooth, and stable layers
• Superior and reproducible performance batch-to-batch and plate-to-plate

Rediscover the Power of Thin Layer ChromatographyThin layer chromatography (TLC) may be one of the most undervalued methods of chromatographic analysis for the separation and identification of chemical substances.

Thin Layer Chromatography plates provide:

• Rapid results and inexpensive operation
• Qualitative data for compound identification and purity
• The ability to monitor the progress of chemical reactions
• Aid in developing optimal methods
• Quantitative data with the proper instrumentation

Benefits of TLC Plates

TLC is the chromatographers’ fast-lane choice for:

• Ultra-fast solvent and gradient optimization prior to HPLC
• Determining the number of major compounds present in a mixture
• Quick assessment and monitoring of both biotransformations and synthetic reactions
• Choosing the appropriate solvent system for column and flash chromatography
• Simultaneous analysis of several samples under identical conditions

Enhanced quality control & extensive product portfolio

Our TLC plates are manufactured with stringent production controls. TLC layers are smooth, homogenous, uniform, and reproducible from plate-to-plate and lot-to-lot.

TLC ready-to-use layers coated with hydrophilic silica have been in use for a number of years, and the majority of all TLC separation challenges can be solved with these layers. It is to your advantage to make certain separations on lipophilic silica layers, i.e. in reversed phase. Due to the rapid development of HPLC columns, there is an increasing demand for TLC plates which can be used for comparing the expected outcome of a HPLC chromatograph. In HPLC, reversed phase systems based on C8 or C18 modified silicas are frequently used. These systems are suited for the separation of very polar (hydrophilic) classes of compounds. Prior to the introduction of those reversed phase systems, polar compounds were separated by ion exchange chromatography. Reversed phase TLC with alkyl modified silicas as stationary phases opens many doors on potential TLC applications.

HPTLC plates C18-50 (50% silanization) and C18-100 (100% silanization). Sorbent Technologies provides C18 silane for our reversed phase TLC plates, but with varying levels (percentages) of silanization.

We offer reversed phase plates comprising of a medium pore size silica (mean pore diameter 60 Å), with a mean particle size of 2-10 µm (identical to the material used for our HPTLC silica plates). This HPTLC silica is reacted to a different degree, either totally (= 100%) or partially (= 50% of the reactive silanol groups). This unique modified silica has found several successful applications in anhydrous eluents as well as eluents with high concentrations of water can be used (see Table 1).

As fluorescent indicator, we use an acid-resistant product which allows application of strongly acidic eluents without undesirable fluorescence quenching. This fluorescence indicator emits a pale blue fluorescence in short-wave UV light. UV absorbing substances appear as dark-blue to black spots on a light-blue background.

The two available layers differ by their selectivity, possibly due to a combination of hydrophobic and polar interactions. Table 1 clearly demonstrates differences in migration distances for different contents of water, which indicates differences in wettability.

Table 1: Benefits of Using a Wettable Layer:
Migration of C18-50 and C18-100 silica layers as compared to C18-W Plates

Chromatography > Thin Layer Chromatography (TLC) > Normal Phase TLC Plates > C18-W TLC Plates